Analysis Note

ControlMouse brain lysate

Application

Research CategoryNeuroscience

This Anti-Glutamate receptor 1 Antibody is validated for use in WB, IH(P), IP for the detection of Glutamate receptor 1.

Research Sub CategoryNeurotransmitters & Receptors

Immunohistochemistry Analysis: 1:300 dilution of this antibody has been shown to detect Glutamate receptor 1 (GluR1) in rat brain tissue.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Glutamate receptors (GluRs) can be categorized as ionotropic or metabotropic and subcatergorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. In general, AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition. GluR1 is believed to mediate calcium influx, and has a vital function in synaptic plasticity related to learning. Reduction in GluR1 has been caused by FMR1 gene mutation and contributes to fragile X syndrome. Changes in expression of GluR1 receptors on dopamine neurons have been correlated with sensitization of drugs of abuse.

Immunogen

Epitope: Cytoplasmic domain

KLH-conjugated linear peptide corresponding to human Glutamate receptor 1 at the cytoplasmic domain.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in mouse brain lysate.

Western Blot Analysis: 1:1000 dilution of this antibody detected Glutamate receptor 1 (GluR1) on 10 µg of mouse brain lysate.

Specificity

This antibody recognizes Glutamate receptor 1 at the cytoplasmic domain.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

Observed at ~106 kDa